Introduction The purpose of this lab was to identify unknown bacteria cultures using various differential tests, and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were Gram stain, Catalase, Mannitol Salt Agar (MSA), Blood Agar, Novobiocin, Coagulase, and DNAse (Alachi, 2007). Rebekah Worley February 21, 2012 Mitchell Section 4 Biol 311 Staining and …show more content…
This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped others make antibiotics used today. This lab was completed by using the methods learned thus far in identification of bacteria. There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics to other purposes such as knowing the exact microorganism has to be used to make certain foods. This experiment was done by applying methods in order to identify an unknown bacterium. An unknown bacterium was handed out by the lab instructor. The methods that have been learned so far in identifying bacteria were applied to this unknown. Procedures were followed as stated in the lab manual and biochemical test handouts. The first procedure that was done was a gram stain followed by a streak of the unknown on a TSA plate in order to determine the gram reaction and observe the colony morphology. After that, specific biochemical tests were performed for gram positive, since unknown number five was determined to be gram positive rod. The other tests were performed in this order: Mannitol Salt (MSA) streak, Blood Agar streak, Catalase test, Nitrate Reduction test, and Phenyl
And the unknown bacterial sample for Group 11 is… UNKNOWN. We were unable to identify our bacteria due to cross-contamination at some point during our research. This was first brought to our attention while running growth tests (Table 1). At that time, we discovered that most of the tests (EMB-lactose, PEA, Forms String in KOH) were showing that our sample was Gram-Positive, one test (Vancomycin) was showing that there was a Gram-Negative Bacteria growing.
The purpose of this study project was to carefully isolate and identify two unknown bacteria from a mixed culture. The ability to properly evaluate biochemical test results is also necessary for the identification to be successful. The goal was to apply all of the methods and techniques that have been learned in the microbiology laboratory course for the proper identification of unknown bacteria. A certain amount of bacteria that were used throughout the course were possible bacteria that could be found in a mixed culture. The bacteria that were identified in the mixed culture were Staphylococcus Aureus and Kocuria Rhizophila.
This experiment was conducted to find the genus and species of an unknown bacteria prescribed by the lab teacher, which was unknown bacteria GA3 in my case. Identification of unknown bacteria techniques are used on an every day basis to figure out what type of bacteria it is and to find the best method of how to treat a patient with this bacteria (1). All five “I’s” of Microbiology were used in the testing for the unknown culture. Inoculation was used several times to put the unknown culture into agar plates or into biochemical test tubes. After Inoculation of these tubes or plates, they always were placed into the incubator for further growth and development. Isolation was used to make sure we got the correct bacteria we were testing for. After each further isolation, we gram stained the culture and inspected the culture under a microscope to further help in the identification process of the unknown bacteria. Multiple tests were done on the unknown culture to make sure we were confident in what kind of bacteria the unknown was.
The purpose of this study was to identify the unknown bacterium using biochemical tests and various methods that had been learned from previous the microbiology laboratory class. Identifying the unknown bacterium was determined by separating and differentiating possible
An unknown bacterium 15 was awarded and labeled at the table ready to be identified. Using the skills and test that are taught and learned in microbiology were applied into learning what the unknown bacteria culture was. There were multiple procedures and test done in order to gain all the information needed to determine which bacteria was given. In order to find what the bacteria was the first step was finding the right environment and temperature that would allow the bacteria to thrive and grow. Determining this is one of the most important steps in being able to obtain conclusive results that would allow the results of the test to be accurate and correct. Without the correct temperature and environment the bacteria will give inconclusive results which will alter and skew the end results and may lead to the wrong conclusion. By using the methods that were obtained and learned through the microbiology class allowed the skills and knowledge to determine the bacteria and execute the tests in order to determine the culture.
The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed, provided us with some key information about the unknown microbes in question and how the bacteria function.
During the final experiment for Microbiology will have to figure out the unknown organism given to us. The organisms will be narrowed down by using skills we have learned throughout the lab this quarter and the test results will narrow down to a single organism. The reason to perform experiments that determines the identity of bacterium is to find out how to treat it or what infections it is causing. In a controlled environment, students learned how to use various methods to determine how to differentiate between multiple bacteria. This lab report will be detailed steps explaining how to determine the identity of my unknown organism number 12.
After many performed test such as the API 20E test strip, motility test, inoculated plates, gram stain, an identification flow chart which began with the results from an indole, MR, VP, and citrate test. This amongst many other test. These results brought the conclusion of my unknown culture to be Escherichia coli, my unknown number was 11.
In conclusion, after conducting previously mentioned biochemical tests in order to identify the unknown bacteria, became obvious that unknown gram negative was Proteus Vulgaris. Having eliminated all the bacteria that didn’t show expected results and confirming with such tests as catalase, glucose, indole, citrate, urea, and SIM, it was accurate to name the unknown gram negative. Furthermore, gram positive became obvious it was Streptococcus Pneumoniae. Having eliminated all the bacteria that didn’t show expected results and confirming with such tests as blood agar, catalase, and optochin and bacitracin tests, it was accurate to name the unknown gram positive. I have learned that it is extremely important to be to able to identify what kind
The study of two unknown microorganisms was completed by using methods taught in the microbiology laboratory. The basis of the study was identification of those two microorganisms. Identification of the microorganisms is important in many aspects of medicine. Some of these include source of infection, proper treatment, and which antibiotics are effective against the microorganism present.
In class, we were given the task of identifying an unknown bacterium broth culture. After receiving number 69, I went through several tests to figure out what bacterium I received. First, I created a slide from my broth by putting a small amount of the unknown broth on to a clean slide and letting it dry for ten minutes. After this, I stained the slide by applying four reagents in order; crystal violet, grams iodine, decolorizer and safranin. From the stained slide, I discovered that this bacterium was gram-negative, which would determine the next couple of tests I would do to identify my unknown bacterium. I began by streaking for confluent growth from my broth culture onto a TSA plate. From the TSA plate, I aseptically transferred a loop
: In the field of microbiology, there are times when a sample will contain more than one species of bacteria. The goal is to separate each bacterium and culture them independently from one another to identify them. This was the objective of this lab. Each stock contained two unknown bacterium, and the possible unknowns were Eserichia coli, Enterobacter aerogenes, Enterobacter cloacae, Serratia marcescens, Proteus vulgaris, Klebsiella pneumoniae, Shigella flexneri, Shigella sonnei, and Salmonella enterica. The tests available were MacConkey agar, Endo agar, Hektoen Enteric agar, Tryptone Soya Agar (TSA), carbohydrate sugar broths, Triple Sugar Iron (TSI) agar, decarboxylase broths (arginine, lysine, and ornithine), Simmon’s Citrate Agar, urease
Bacteria are identified systematically by using various different techniques and narrow down their species present in an unknown bacterial culture. This is highly usefully in identifying infections correctly and in research regarding microorganism. Laboratories use identification techniques based on primary characteristics and gram stains to carefully identify unknown organism in samples collected (faeces, blood, sputum, mucosal swabs), to determine the cause of infection and define a disease.
It is important for one to understand how microorganisms function as well as their identity. Identifying unknown microorganisms are beneficial to both scientist and patients. Reason being is due to bacterium capable of becoming resistant to antibiotics over a period of time. In order for doctors to treat patients to the best of their ability it is vital that they are able to identify the bacteria first in order to provide proper treatment. In order to identify the two unknown bacterium proper procedures were done. This study was done by applying all of the necessary methods that have been learned thus far in the microbiology laboratory.
A Gram stain was used to determine the morphology of the bacteria and to confirm that the bacteria were Gram-negative. After all of the differential media were inoculated, incubated and observed, unknown bacteria number 20 was determined to be Klebsiella pneumoniae. Introduction When bacteria are obtained from the environment, one needs to perform many differential tests on the bacterial species to identify it. First, the bacteria need to be obtained in pure colonies.