Answered: 1. While working with Mannitol Salt…

1. While working with Mannitol Salt Agar you notice the growth of staphylococci however other microorganisms are growing as well. How would you interpret

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter19: Biomedical Laboratory Instrumentation

Section: Chapter Questions

Problem 4Q

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Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.

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1. While working with Mannitol Salt Agar you notice the growth of staphylococci however other microorganisms are growing as well. How would you interpret this?

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while working with mannitol salt agar you notice the growth of staphylococci however other microorganism are growing as well. how would you interpret this?
While working with Mannitol Salt Agar you notice the growth of staphylococci however other microorganisms are growing as well. How would you interpret this? Explain.
With your tests you now figured out that your patient has a Staphylococcus infection, and you would like to know if the infection is caused by Staphylococcus aureus or by Staphylococcus epidermis. What test can you do next to see if your patient indeed has a Staphylococcus aureus infection? O You can isolate the pathogen from the patient and grow it on a mannitol salt agar. Staphylococcus aureus can ferment mannitol turning the agar plate yellow. Staphylococcus erpidermis cannot ferment mannitol and the agar plate stays red. O You can isolate the pathogen from the patient and grow it on a mannitol salt agar. Staphylococcus epidermis can ferment mannitol turning the agar plate yellow. Staphylococcus aureus cannot ferment mannitol and the agar plate stays red. O There is no way you can distinguish between Staphylococcus aureus and Staphylococcus erpidermis O you do a Gram stain. Staphylococcus aureus will stay purple as it is Gram positive and Staphylococcus erpidermis will show up as…
1.What advantage(s) does the pour plate method have over the streak-plate method? 2.Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the inoculation? 3.Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled to 50° C? 4.Explain why plates should be inverted during incubation. 5.Explain why plates should be inverted during incubation.
In spread plate method, colonies form within the agar and agar surface. In pour plate method, previously prepared agar plates are not required. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.
1. What is one advantage of utilizing the pour plate technique over the streak plate technique ? 2. Why must the agar pours be cooled to 45C before use in the pour plate technique? 3. Explain the consequences if a group removed all the agar pours from the water bath at one time and allowed them to sit on the bench for several minutes before using them. 4. Why can the agar pour tubes be rinsed in the sink after the agar is transferred to the Petri plate ? Could you rinse the tubes if the bacteria had been pipetted into the agar pour tubes rather than in the plates? Explain. 5. What would be the result if a student dipped his / her loop in the stock culture during inoculations of each quadrant ? Explain . part B 1. The introduction stated that microbes are mechanically separated or diluted over the surface of the medium . How is this accomplished ? 2. Go to https://commons.wikimedia.org/wiki/File:MacConkey_agar_with_LF_and_LF_colonies . . Which side of the plate (left or right)…
1. You were given a mixed nutrient agar broth culture of bacteria. a. How will you determine the different types of bacteria present in the mixed culture? b. How will you make a pure culture of these bacteria in a slant nutrient agar? c. How will you identify these bacteria?
1. The differential aspect of MacConkey agar allows for the determination of which bacteria are lactose fermenters and which are not. 2. Blood agar is used to determine an organism's ability for hemolysis. Blood agar is differential in this regard. 3. EMB agar uses two dyes, eosin and methylene blue, to restrict the growth of gram-positive organisms, making this agar (EMB) in this regard. 4. Crystal violet dye in MacConkey agar will prevent some organisms from growing, making MacConkey agar in this regard. The crystal violet dye can also be used in the determination of the ability to ferment the carbohydrate in the agar; making MacConkey agar in this regard. 5. Use of an agar which contains an elevated amount of salt would allow some organisms to grow while preventing the growth of other organisms. This media would be considered selective
1. What does MacConkey select for? How does it select these particular bacteria? What will not grow on a MacConkey agar plate?
1. Which of the following micro-organisms are the most resistant to heat sterilization? a. Vegetative bacteria (e.g., Samonella spp.) b. Spore-forming mesophilic bacteria (e.g., Clostridium botulinum) c. Spore-forming thermophilic bacteria (e.g., Bacillus stearothermophilus) d. Toxicogenic bacteria (e.g., E. coli O157:H7) e. All of the above are equally resistant to heat sterilization.
1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.
1. What is the purpose of adding mineral oil to an O/F Media tube and why do we only put mineral oil in only one? 2. Which sugars are typically tested in the phenol red broth? Can the phenol red test be also used to determine if a certain bacterium can metabolize various carbohydrates? 3. Explain how conducting the MR/VP test too soon in a culture's growth cycle can lead to false negatives? 4. Generally, the color change we see in these tests is a result of what change in the media? Explain. 5. How does the production of urease acid aid Helicabactor. pylori ability to live in the stomach?