Create the map for question #11

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Create the map for question #11
Question #8 and # 10 already answered:
8. Draw two separate lines representing the two fragments for the Ava II digest in the space below.
Give the size (in Kb) of each fragment AND label both ends for both fragments as Ava II digest
sites.
Ava I
4
2.99 кв
takb
F
Avd
++
Bu
4 kb
#
Puu
1
ANG II
10. Draw and label these Pvz II sites on the appropriate Ava II fragment above (in question 8)
and indicate their distance from adjacent recognition sites, in Kh, by indicating the sizes of the
fragments they create when digested by both Pru II and Ava II. HINT: think about your answer
to question 7 again!
Starting wells (origin) →
red: kb
black cm
=
Solve #11 using the information provided
11. Now join the 2 fragments from question 8, above, to recreate the original pUC 19 plasmid.
Draw this circular map on the Figure below starting with the Ava II site arbitrarily placed at the top
of the circle. Be sure to LABEL the remaining recognition sites AND indicate their location with
the distance in Kb between adjacent recognition sites (the way you should have in the map on p.
8). Hint: make sure your map would produce the fragments shown in Figure 5, lane 5, if the
double digests were repeated!
Figure 5.
Results of gel electrophoresis.
Marker lengths given in Kb.
Measure and record next to each
band the sistance traveled, as
measured from the origin. Also
record the sizes for bands in lanes
3-5 as determined using the
procedure above and the graph on
p. 10.
0.14 kb
Lane # 1
Figure 5 (includes lane 5):
1. Кь
Ava II
PUC 19
Empty Lambda Mol
lanc
weight
markers
2.322
2.027
2
9.416
6.557 T
4.361
0.564
0.125
52
(-) electrode
3
Avail
digest
O
4
0.140
Pwll
digest
O
2.98 25 25.5
9/³
0.240
(+) electrode
5
Avali/
Pull
doest
O
0.76
0-240
0-140
Transcribed Image Text:Question #8 and # 10 already answered: 8. Draw two separate lines representing the two fragments for the Ava II digest in the space below. Give the size (in Kb) of each fragment AND label both ends for both fragments as Ava II digest sites. Ava I 4 2.99 кв takb F Avd ++ Bu 4 kb # Puu 1 ANG II 10. Draw and label these Pvz II sites on the appropriate Ava II fragment above (in question 8) and indicate their distance from adjacent recognition sites, in Kh, by indicating the sizes of the fragments they create when digested by both Pru II and Ava II. HINT: think about your answer to question 7 again! Starting wells (origin) → red: kb black cm = Solve #11 using the information provided 11. Now join the 2 fragments from question 8, above, to recreate the original pUC 19 plasmid. Draw this circular map on the Figure below starting with the Ava II site arbitrarily placed at the top of the circle. Be sure to LABEL the remaining recognition sites AND indicate their location with the distance in Kb between adjacent recognition sites (the way you should have in the map on p. 8). Hint: make sure your map would produce the fragments shown in Figure 5, lane 5, if the double digests were repeated! Figure 5. Results of gel electrophoresis. Marker lengths given in Kb. Measure and record next to each band the sistance traveled, as measured from the origin. Also record the sizes for bands in lanes 3-5 as determined using the procedure above and the graph on p. 10. 0.14 kb Lane # 1 Figure 5 (includes lane 5): 1. Кь Ava II PUC 19 Empty Lambda Mol lanc weight markers 2.322 2.027 2 9.416 6.557 T 4.361 0.564 0.125 52 (-) electrode 3 Avail digest O 4 0.140 Pwll digest O 2.98 25 25.5 9/³ 0.240 (+) electrode 5 Avali/ Pull doest O 0.76 0-240 0-140
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