3. You use the external standard technique to get the following function for a linear regression, y = (0.5423 AU/(ug/mL))*x + 0.0354 AU. The unknown you measure has an absorbance of 0.237 AU. What is the concentration of the unknown sample?
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The answer is 0.372 ug/mL
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- The response of the instrument must be lincar in the sample matrix. Systematic errors can occur due to sample or standard preparation. It consists of a series of standard analyte solutions separate from the unknown. A blank is used to correct the response from the instrument. Known quantities of a standard solution of the analyte are added to the unknown. It is the method of choice when the matrix of the sample may affect the signal. Matrix effects can cause the same analyte concentration in an unknown and standard to give different responses. It is used to measure the instrument response as a function of a known analyte concentration under a set of conditions.A solution was prepared from an amount n = (0.500 ± 0.005) mol dissolved in a volume V = (250.0 ± 0.5) cm3. What is the absolute uncertainty in the concentration in units of mol dm-3?A student prepared a cereal sample solution using a procedure similar to that in Lab 5. The original sample solution was diluted by a factor of two, and then the absorbance of the diluted sample solution was measured and recorded as (2.0x10^-1). Use the following calibration plot and the dilution factor to determine the concentration of iron, in unit of M, in the original cereal sample solution. The trendline (linear regression) as displayed on the plot is y = 11898x - 0.0112.
- What is a 99% confidence interval uncertainty of the slope when we are given a trendline with a slope of 42.211 and an R² value of 0.9991 for a set of 145 data points?B2. During a mass spectrometry experiment, the spectrum of vascular endothelial growth factor A (VEGF-A) was acquired. The absolute error of VEGF-A, having a mass of 1024.5210 Da was 0.06 Da. Calculate the relative error (ppm).In a Bradford assay, 11 µl of a protein isolate sample was diluted by adding 21 µl of water prior to the addition 2.0 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original protein isolate sample whose absorbance reading at 595 nm is 0.304 after dilution. The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose concentration is 200 µg/mL. The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table below: Standard soln # 1 2 13 4 6 conc of BSA 12.00 24.00 36.00 48.00 60.00 (pg/mL) Absorbance at 595 nm 0.000 0.156 0.249 0.328 0.411 0.528 zNote: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of the calculation.
- In spectrophotometric analysis, the relationship between the absorbance A and the analyte's concentration C is: A = ebC + Ablank What is the molar extinction coefficient I and its absolute uncertainty if A is 0.68 ± 0.06; Ablank is 0.06 ± 0.01; b is 1.00 cm; and C is (0.45 ± 0.03)x10-5 mol/L?The concentration of hydronium ions and hydroxide ions can vary widely in a solution, easily from 1 M to 1 x 10-14 M. This variation is too wide to fit on an analog readout. In order to make this into a smaller scale suitable for an analog readout, the log to the base 10 was used so that the read out is focused on powers of 10. Note that the log [1] = 0, and the log of 1 x 10-14 = -14. It was then decided that the numbers should be positive so the sign was changed. The way to measure concentrations of both H+ and OH- was thus defined as -log. This was then referred to as p. Thus, pH = -log[H+], pOH = -log[OH-], and pK = -log[Keq]. You need to figure out how to do this mathematical manipulation on your calculator. The acid-base reaction in pure water is H2O + H2O ⇔ H3O+1 + OH-1 and it was found that in pure water under standard conditions the [H3O+1] = [OH-1] = 1 x 10-7 M Neutral pH is defined as having a [H+] or [H3O+1] of 1 x 10-7 M. The pH of a neutral solution is thus pH = -log[1 x…Determine the slope of the linear regression line for a standard curve of concentration versus absorbance using the following data. Concentration (M) Absorbance 2.00x10-5 0.0500 5.00x10-5 0.130 1.00x10-4 0.200 2.00x10-4 0.450 5.00x10-4 1.25 7.50x10-4 1.40 Group of answer choice 5.00x10-4 1.00x10-5 1990 0.0420 0.456 0.296
- cont irmation True or false: The purpose of the internal standard is to make quantitation of the analyte insensitive to injection errorsThe standard curve of a biuret assay gave the equation: y=0.4193x. The absorbance for Unknown A = 0.098 and Unknown B = 0.325. Are you equally confident in the accuracy of the protein %D concentrations you would calculate for samples Unknown A and Unknown B? Briefly explain your answer.(2) A CHEM 215 student performed 5 measurements of the wt% of SO4²- of a solid power sample. The results are 8.75%, 8.85%, 8.89%, 8.71%, 8.64%. The standard deviation is 0.10%. What is the confidence interval for this student's experiment at 95% confidence level. The t values for 95% confidence for degree of freedom from 1 to 5 are 12.71, 4.303, 3.182, 2.776, 2.571, respectively. Show your work!
