A 12 kb linear DNA fragment is subject to single or double RE digest and agarose gel electrophoresis, to yield the gel profile shown below. The first lane contains the size marker (M). a) Explain how the name of the enzyme EcoRI is derived. b) How many sites are there for EcoRI and PvuII respectively on this DNA fragment? c) Use the sizes of the DNA bands on the gel to compile a restriction enzyme map of the DNA fragment. Indicate the positions of the restriction enzymes sites for EcoRI and PvuII on the map.
A 12 kb linear DNA fragment is subject to single or double RE digest and agarose gel electrophoresis, to yield the gel profile shown below. The first lane contains the size marker (M). a) Explain how the name of the enzyme EcoRI is derived. b) How many sites are there for EcoRI and PvuII respectively on this DNA fragment? c) Use the sizes of the DNA bands on the gel to compile a restriction enzyme map of the DNA fragment. Indicate the positions of the restriction enzymes sites for EcoRI and PvuII on the map.
Essentials Health Info Management Principles/Practices
4th Edition
ISBN:9780357191651
Author:Bowie
Publisher:Bowie
Chapter4: Introduction To The Patient Record
Section: Chapter Questions
Problem 1CR
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A 12 kb linear DNA fragment is subject to single or double RE digest and agarose gel
electrophoresis, to yield the gel profile shown below. The first lane contains the size marker
(M).
a) Explain how the name of the enzyme EcoRI is derived.
b) How many sites are there for EcoRI and PvuII respectively on this DNA fragment?
c) Use the sizes of the DNA bands on the gel to compile a restriction enzyme map of the DNA
fragment. Indicate the positions of the restriction enzymes sites for EcoRI and PvuII on the
map.
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