A piece of DNA 5.0 kb long is cloned and then cut out of the vector for analysis. This linearpiece of DNA is digested with two restriction enzymes, EcoRI and BamHI, individually and incombination, and the resulting fragment sizes are determined by electrophoresis. The results areas follows:Restriction fragment size4.5 kb; 0.5 kb3.0 kb; 2.0 kb2.5 kb; 2.0 kb; 0.5 kbEnzyme nameEcoRIВатHIEcoRI + BamHIConstruct a potential restriction map based on these results.

Restriction enzyme is any enzyme that cleaves DNA at a particular sequence. Example - BamHI, EcoRI,…

Answered: A piece of DNA 5.0 kb long is cloned…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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All of the following are performed during restriction fragment length polymorphism analysis. 1. splitting of double-stranded into single-stranded DNA 2. gel electrophoresis 3. autoradiography 4. immersion in radioactive probes 5. digestion of DNA with restriction endonucleases 6. use of a positive charge to transfer single-stranded DNA from a gel to a membrane. The correct sequence of these operations is what
As part of the genomic analysis of "Maxbacterium", one of the small DNA circles was digested with EcoRI and Hind III restriction endonuclease enzymes. Electrophoresis of the resulting digestion fragments is shown in the gel below to help student groups construct the restriction map in their breakout room. Size of EcoR1 HindIII EcoR1 + Fragment digest digest HindIII digest Origin 17kb 10kb 7kb 6kb 1kb Based on the banding pattern and constructed map which of the following statements is TRUE? O A. The largest fragment in the restriction map is 17KB. O B. The information provided is enough to quantify the amount of DNA in the gel. O C. The migration on the gel depends on the proportion of AT pairs in the DNA. O D. The constructed restriction map has three cut sites.
A 22-kb piece of DNA has the following restriction sites:A batch of this DNA is first fully digested by HpaI alone, then another batch is fully digested by HindIII alone, and finally, a third batch is fully digested by both HpaI and HindIII together. The fragments resulting from each of the three digestions are placed in separate wells of an agarose gel, separated by gel electrophoresis, and stained by ethidium bromide. Draw the bands as they would appear on the gel.
Below is a plasmid with restriction sites for Baml and EcoRI, Several restriction digests were done using these two enzymes either alone or in combination. Hint: Begin by determining the number and size of the fragments produced with each enzyme. "kb" stands for kilobases, or thousands of base pairs. Plasmid Gel lanes I | III IV V 6 Kb Bam HI Bam HI. 20 Kb PGEN101 (20 Kb) 2 Kb 11 Kb Bam HI 8 Kb 8 Kb 4 Kb 6 Kb Eco RI 3 Kb 21. Which lane shows a digest with BamHl only? a. I b.I c. II d. IV e. V 22. Which lane shows a digest with EcoRLonly? a. I 23. Which lane shows the fragments produced when the plasmid was incubated with both EcoRI and BamH1? a. I b.I c. II d. IV e. V b. I c. II d. IV e. V Base pairs
When the restriction endonuclease EcoRI is used to digest a 10 kb DNA fragment, it produces 4 kb and 6 kb-sized fragments. Digesting the 10 kb fragment with BamHI yields three fragments, each ranging in size from one to three and a half kilobytes. Four pieces of 0.5, 1, 3 and 5.5 kb are formed after using both enzymes. Create a restriction map for this 10 kb piece of DNA using the information you have collected. Make a note of where the two enzymes cut, as well as the distances between the enzymes.
Consider the following image of a stained agarose gel representing the separation of a plece of DNA that has been digested by a series of restriction enzymes. Assume no digest fragment is of identical size to another digest fragment. nucleotide pairs (kb) 8 S 45 3.5 AA size Hindill + markers EcoRI Hindill EcoRI - 1st attempt Based on all data presented in the stained agarose gel, which of the following statements is correct? Choose one: OA. The original piece of DNA was circular and was cut by EcoRI twice and Hindill only once. OB. The original piece of DNA was linear and was cut by EcoRI twice and Hindill only once. OC. The original piece of DNA was circular and was cut by EcoRI once and not at all by Hind!!!.
A small DNA molecule was cleaved with several different restriction nucleases, and the size of each fragment was determined by gel electrophoresis. The following data were obtained.
The human gene for hemophilia is on the X chromosome. Below is a pedigree from a family afflicted with hemophilia. The man with blackened symbol (III-1) has hemophilia. To help with genetic diagnosis, a probe that detects an RFLP (restriction fragment length polymorphism) on the X chromosome is used. This probe detects either a 7 kb restriction enzyme fragment or 3 kb and 4 kb restriction enzyme fragments. The RFLP pattern for all the members of the pedigree is shown. The recombination distance between the RFLP and the hemophilia locus is 40%. What is the chance that their unborn child (III-7) is affected? 4 kb 3 kb Select one: a. 10% b. 20% C. 40% d. 60% e. 80% f. 90% g. None of these above II II
A result of agarose gel electrophoresis of plasmid DNA is shown below. The direction of the electrophoresis is indicated by the arrow head. Lane 1 shows the plasmid DNA with no restriction enzyme digestion. Lane 2 shows the plasmid DNA which has been digested by restriction enzyme ECORI. Given the fact that there are only relaxed and supercoiled forms existing in this plasmid, please annotate the bands in Lane 1 on this gel with their supercoiling status (supercoiled or relaxed) and explain why. Then based on the information from Lane 1 and Lane 2, analyze the number of EcoRI cleavage sites on this plasmid. Lane 1 2
The gene you are asked to clone is 30,000 bps in length. When you are choosing a suitable Restriction Endonuclease, what criteria about the enzyme can you deduce from the gene length?
A group of overlapping clones, designated A through F, is isolated from one region of a chromosome. Each of the clones is separately cleaved by a restriction enzyme, and the pieces are resolved by agarose gel lectrophoresis,with the results shown below. There are nine different restriction fragments in this chromosomal region, with a subset appearing in each clone. Using this information, deduce the order of the restriction fragments in the chromosome.
A restriction map lists the locations of DNA sequences that are cut by a particular restriction enzyme for a piece of DNA, such as a chromosome or a plasmid. Restriction maps are important when generating a construct for experimental use. Digesting the DNA sequence with the restriction enzymes will result in fragmented DNA of predictable sizes, based on the restriction map, that allow a researcher to analyze if his or her construct was generated correctly when visualized using gel electrophoresis. Use the linear restriction map to predict where bands would be expected on a gel if a digest is performed using the specified restriction enzymes. Assume that there is enough restriction enzyme that every possible restriction site on each molecule of DNA will be cut.

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