An electrophoresis experiment is performed with a sample containing the amino acid derivative shown. The pKa of the carboxylic acid group is 2.4, the pKa of the protonated amine group is 9.6, the pKa of the side chain as shown is 3.8. Describe where the amino acid derivative will migrate depending on the properties of the buffer used. Make a sound and complete argument to defend your response.

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Chapter1: Chemical Foundations
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An electrophoresis experiment is performed with a sample containing the
amino acid derivative shown. The pKa of the carboxylic acid group is 2.4, the
pKa of the protonated amine group is 9.6, the pKa of the side chain as shown
is 3.8. Describe where the amino acid derivative will migrate depending on
the properties of the buffer used. Make a sound and complete argument to
defend your response.
HO
H₂N
OH
Transcribed Image Text:An electrophoresis experiment is performed with a sample containing the amino acid derivative shown. The pKa of the carboxylic acid group is 2.4, the pKa of the protonated amine group is 9.6, the pKa of the side chain as shown is 3.8. Describe where the amino acid derivative will migrate depending on the properties of the buffer used. Make a sound and complete argument to defend your response. HO H₂N OH
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