Exactly 5.00 mL aliquots of a solution containing analyte X were transferred into 50.00-mL volumetric flasks and the pH of the solution is adjusted to 9.0. The following volumes of a standard solution containing 2.00 µg/mL of X were then added into each flask and the mixture was diluted to volume: 0.000, 0.500, 1.00, 1.50 and 2.00 mL. The fluorescence of each of these solutions was measured with a fluorometer, and the following values were obtained: 3.26, 4.80, 6.42, 8.02 and 9.56, respectively. ii. Using relevant functions in Excel, derive a least-squares equation for the data, and use the parameters of this equation to find the concentration of the phenobarbital in the unknown solution.
Exactly 5.00 mL aliquots of a solution containing analyte X were transferred into 50.00-mL volumetric flasks and the pH of the solution is adjusted to 9.0. The following volumes of a standard solution containing 2.00 µg/mL of X were then added into each flask and the mixture was diluted to volume: 0.000, 0.500, 1.00, 1.50 and 2.00 mL. The fluorescence of each of these solutions was measured with a fluorometer, and the following values were obtained: 3.26, 4.80, 6.42, 8.02 and 9.56, respectively. ii. Using relevant functions in Excel, derive a least-squares equation for the data, and use the parameters of this equation to find the concentration of the phenobarbital in the unknown solution.
Chemistry: Principles and Reactions
8th Edition
ISBN:9781305079373
Author:William L. Masterton, Cecile N. Hurley
Publisher:William L. Masterton, Cecile N. Hurley
Chapter14: Equilibria In Acid-base Solutions
Section: Chapter Questions
Problem 77QAP: Two students were asked to determine the Kb of an unknown base. They were given a bottle with a...
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Question
![Exactly 5.00 mL aliquots of a
solution containing analyte X were
transferred into 50.00-mL
volumetric flasks and the pH of
the solution is adjusted to 9.0. The
following volumes of a standard
solution containing 2.00 µg/mL of
X were then added into each flask
and the mixture was diluted to
volume: 0.000, 0.500, 1.00, 1.50
and 2.00 mL. The fluorescence of
each of these solutions was
measured with a fluorometer, and
the following values were
obtained: 3.26, 4.80, 6.42, 8.02
and 9.56, respectively.
ii. Using relevant functions in
Excel, derive a
least-squares equation for
the data, and use the
parameters of this
equation to find the
concentration of the
phenobarbital in the
unknown solution.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F6e368e70-ff1e-4ae8-8d02-323e88dc5b0c%2F7277f0de-bd51-4409-bd07-fdbe5e715fd5%2F4frpyy_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Exactly 5.00 mL aliquots of a
solution containing analyte X were
transferred into 50.00-mL
volumetric flasks and the pH of
the solution is adjusted to 9.0. The
following volumes of a standard
solution containing 2.00 µg/mL of
X were then added into each flask
and the mixture was diluted to
volume: 0.000, 0.500, 1.00, 1.50
and 2.00 mL. The fluorescence of
each of these solutions was
measured with a fluorometer, and
the following values were
obtained: 3.26, 4.80, 6.42, 8.02
and 9.56, respectively.
ii. Using relevant functions in
Excel, derive a
least-squares equation for
the data, and use the
parameters of this
equation to find the
concentration of the
phenobarbital in the
unknown solution.
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