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- 1.The study of Introduction to Microbiology showcases the enormous impact of microbes on living organisms and the environment at large. Based on your understanding, discuss five ways by which the human societal setting is impacted by microorganisms and microbiology on the whole 2.Explain any four ways that the knowledge acquired from introduction to microbiology can be applied in the current fight against the current COVID-19 pandemic raging havoc against the human population.What is the BEST glassware that could be used to hold the agar powder while weighing? After 24 hours, growth can be seen from your plates. If you want to put dyes on your sample, what rack is used?5. Complex and enriched media are most ideal to identify specific microbes from a mixed population. True or False
- explain how the following genotypic andphenotypic methods of microbe identification is done. Use diagrams or schematic representations to explain yourwork. 1.Gram Staining2.Biochemical Reactions: Multiple Tube Fermentation Technique3.(Biochemical Reactions: IMViC Test1. Identify the test/stain in Microbiology 2. Indicate the observations of a positive result and a negative result for each test/stain 3. Explain what each positive result and each negative result mean interpretation for each test/stain 4. Identify the organisms that are removed from your possible identifications at each stage of the flow chart, and which organisms remain as possible identifications at these steps as well2. Why can't we say "sterile" technique? 3. How are aseptic technique similar and different in the lab and healthcare field? Be specific and explain at least two differences and two similarities. 4. You are asked to develop a method to transfer an unknown organism from a liquid broth to a solid petri dish. List each step that you would have to take. Be specific.
- 1. Give 2 systems used at present for the automatic identification of bacteria. 2. Why aseptic technique important in urine collection for culture? 3. What are the specimen consideration you have to remember in preparing blood culture? 4. Being a medical technology student, how can you contribute to avoid water pollution and degradation of natural resources?3. ) what are the applications of microbiology in nursing?1)describe how you can use an ‘agar deep’ to learn more about an unknown microbe. These are sometimes also called ‘semi-solid deeps’.
- What is the goal of aseptic techniques To prevent contamination O To allow contamination all of the above O To reduce contaminationActivity 2. Media Used in Isolating Coliforms You are a group of microbiologists tasked to test the presence of coliforms in the newly built water station. You are asked to isolate E. coli, Salmonella, and Shigella. Enumerate the media used for each step of isolating these bacteria. Include pictures and references. Bacteria Enrichment (Broth/Agar) Presumptive test (Broth/Agar) Isolation Media (Broth/Agar) E. coli Salmonella Shigella 1. How can you confirm that E. coli, Salmonella, and Shigella are present in the newly built water station? Explain. 2. What is the difference between nutrient broth and nutrient agar? 3. What is the importance of these steps (Enrichment, Isolation, and presumptive test) in isolating bacteria? Conclusion about the process of isolating bacteria1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.