Please help me to answer a question. I have 2 mg/mL stock solution and I need to make standards of 2.0, 1.0, 0.5, 0.25, 0.125 and 0 mg/mL. Water is used as diluent. How will make them by a serial dilution? As a result, I will need 50 uL of each sample for the next steps - so my method should produce in excess of 50 µL of each dilution. Please help me to calculate the mass of BSA in each sample too. Your help will be greatly appreciated.

Macroscale and Microscale Organic Experiments
7th Edition
ISBN:9781305577190
Author:Kenneth L. Williamson, Katherine M. Masters
Publisher:Kenneth L. Williamson, Katherine M. Masters
Chapter7: Extraction
Section: Chapter Questions
Problem 1Q
icon
Related questions
Question
Please do it fast
Please help me to answer a question. I have 2
mg/mL stock solution and I need to make
standards of 2.0, 1.0, 0.5, 0.25, 0.125 and 0
mg/mL. Water is used as diluent. How will I make
them by a serial dilution? As a result, I will need 50
µl of each sample for the next steps - so my
method should produce in excess of 50 µL of each
dilution. Please help me to calculate the mass of
BSA in each sample too. Your help will be greatly
appreciated.
Transcribed Image Text:Please help me to answer a question. I have 2 mg/mL stock solution and I need to make standards of 2.0, 1.0, 0.5, 0.25, 0.125 and 0 mg/mL. Water is used as diluent. How will I make them by a serial dilution? As a result, I will need 50 µl of each sample for the next steps - so my method should produce in excess of 50 µL of each dilution. Please help me to calculate the mass of BSA in each sample too. Your help will be greatly appreciated.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

Blurred answer
Knowledge Booster
Green Chemistry
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Macroscale and Microscale Organic Experiments
Macroscale and Microscale Organic Experiments
Chemistry
ISBN:
9781305577190
Author:
Kenneth L. Williamson, Katherine M. Masters
Publisher:
Brooks Cole
Appl Of Ms Excel In Analytical Chemistry
Appl Of Ms Excel In Analytical Chemistry
Chemistry
ISBN:
9781285686691
Author:
Crouch
Publisher:
Cengage
Fundamentals Of Analytical Chemistry
Fundamentals Of Analytical Chemistry
Chemistry
ISBN:
9781285640686
Author:
Skoog
Publisher:
Cengage