The protein content of the unknown samples were determined using BSA as standard. The absorbance values of the standard solutions and samples are as follows. BSA concentration (mg/mL) Absorbance 0.000 20 0.110 40 0.192 60 0.248 80 0.325 100 0.400 120 0.460 What is the equation of the line? b. Determine the protein concentration of samples in µg/mL. Sample dilutions A540 1 in 14 1 in 5 1 in 8 0.150 0.380 0.235

The protein content of the unknown samples were determined using BSA as standard. The absorbance values of the standard solutions and samples are as follows. BSA concentration (mg/mL) Absorbance 0.000 20 0.110 40 0.192 60 0.248 80 0.325 100 0.400 120 0.460 What is the equation of the line? b. Determine the protein concentration of samples in µg/mL. Sample dilutions A540 1 in 14 1 in 5 1 in 8 0.150 0.380 0.235

Chemistry: The Molecular Science

5th Edition

ISBN:9781285199047

Author:John W. Moore, Conrad L. Stanitski

Publisher:John W. Moore, Conrad L. Stanitski

Chapter13: The Chemistry Of Solutes And Solutions

Section: Chapter Questions

Problem 42QRT

See similar textbooks

Similar questions

A 0.1ml serum sample was analyzed for glucose content and found to contain 250 ng. The concentration of glucose is ... ppm. 4.00 400 25.0 0.25 0.4 0.04 0.025 2.50 40.0 250
Concentration of Acetic Acid in a Vinegar Solution. Average molarity of NaOH Volume HC₂H30₂ sample Final buret reading Initial buret reading Volume NaOH (Show all units and all calculations) Molarity of HC₂H30₂ (unrounded) Average molarity of HC₂H302 (unrounded) Deviations Average deviation Best estimate for molarity of HC₂H302 Average mass percent HC₂H302 46.4mL Oml Ran out of Base ↓ ↓ ↓ ↓ 10.014 HOA je beart giov isults weari?! ferottelucuny Cambia) 10 Kousad Question 1. A Rolaids tablet contains calcium carbonate that neutralizes stomach acid. If 44.55 mL of 0.448 M hydrochloric acid is required to neutralize one tablet, how many milligrams of calcium carbonate are in a Rolaids tablet? CaCO, (s) + 2HCl(aq) → CaCl₂ (aq) + H₂O (1) + CO₂ (g)
Twenty sodium salicylate tablets labeled 325 mg were dispersed in sufficient water to make 200.0 ml. A 15.0-ml aliquot of the filtrate was titrated to a bromophenol blue end point in the usual way by 29.11 ml of 0.1000 N hydrochloric acid. Calculate the amount of sodium salicylate in each tablet and, from that, the percentage of the labeled amount. Hint: Use the above formula.
A water sample B has a turbidity reading of 50 NTU. If a 50-ppm standard has a turbidity reading of 100 NTU, what is the concentration of the analyte in A that causes the water to be turbid? a. 50 ppm b.100 ppm c. 75 ppm d. 150 ppm e. 25 ppm
09. Two samples of the same soil is subjected to pH analysis using a pH meter, the first is analysed using 1:1 soil:water ratio and the other with 1:1 soil:KCl ratio, what would be the most probable result? A. Soil analused using 1:1 soil:KCl ratio will register a higher pH compared to that of soil analysed using 1:1 soil water ratio B. Soil analysed using 1:1 soil:KCl will register a lower pH compared to that of soil analysed using 1:1 soil:water ratio C. Soil analysed using 1:1 soil:water ratio will register a lower pH compared to that of soil analysed using 1:1 soil:KCl ratio D. Soil analysed using 1:1 soil:water ratio will have the same pH with that of soil analysed using 1:1 soil:KCl ratio
You are creating a standard curve for a protein experiment. You have to stock solution of BS a and would like to obtain dilutions. Explain how you would create a 1:1000 dilution of your protein stock solution.
w w w w w T Concentration (grams/kg H₂O) 1000 900 800 700 600 500 400 300 200 100 0 0 Solubility Curve for Potassium Dichromate (K₂Cr₂O₂) 10 20 30 40 50 60 70 80 90 100 Temperature (Celsius) Use the solubility curve for questions 22-25. You put 480 g K₂Cr₂O, in 1 kg of water? At which temperature will the 22. solution be saturated? A. 40 °C B. 70 °C C. 1000 °C D. Equal amounts of K₂Cr₂O; will dissolved at all of the listed temperatures. You put 150 g K₂Cr₂O7 in 1 kg of water? At which temperature will the 23. solution be saturated? A. 30 °C B. 50 °C C. 80 °C D. K₂Cr₂O, will dissolved equal fast at all of the listed temperatures.
1. 500.0 mL 0.2 M KI2. 500.0 mL 0.2 M KCl3. 500.0 mL 0.1 M K2S2O84. 500.0 mL 0.1 M K2SO45. 500.0 mL 4.0 mM Na2S2O3 (from Na2S2O3∙5H2O)pls compelete the 2nd table given the data
A 5.04 g sample of a solid containing Ni is dissolved in 20.0 mL water. A 5.00 mL aliquot of this solution is diluted to 100.0 mL and analyzed in the lab. The analyzed solution was determined to contain 5.53 ppm Ni. Determine the molar concentration of Ni in the 20.0 mL solution. concentration: M Determine the mass, in grams, of Ni in the original sample. mass: g Determine the weight percent of Ni in the sample. weight percent: % * TOOLS x10
4. The Ksp for MgCO3 = 1.1 x 101º. CIRCLE the solubility of MgCO3 in pure water. a) 1.1 x 10-10 M b) 1.1 x 108 M c) 1.1 x 107 M d) 1.1 x 106 M e) 1.1 x 10$ M
Saved CHEMY 101 SEC SEM T2 2020-21 The amount of water which should be added to 0.700 M NAOH solution to make 100 mL 0.250 M solution is 19 Multiple Choice 01:25:58 79.6 mL 35.7 mL 55.2 mL 64.3 mL raw K Prev 19 of 20 Next >
A drug is ordered to be administered IV at the initial rate of 4 mU/min, and may increase by 2 mu/min q 15 min to a max of 10 mU/min. The IV strength is 5 mU/mL. Construct the first two lines of the titration table for this order. O A. Dose Rate (mU/min) IV rate (mL/h) 4 mU/min (initial) 8 mU/min 36 mL/h 48 mL/h O B. Dose Rate (mU/min) 4 mU/min (initial) 6 mU/min Oc. Dose Rate (mU/min) 4 mU/min (initial) 6 mU/min O D. Dose Rate (mu/min) 4 mU/min (initial) 6 mu/min IV rate (mL/h) 24 mL/h 48 mL/h IV rate (mL/h) 48 mL/h 72 mL/h IV rate (mL/h) 36 mL/h 36 mL/h