this gene was amplified from each member of the family and run on the gel shown below. The thickness of the band indicates relative amount of DNA. Use the pedigree and the gel to determine the most likely mode of inheritance for this disease. Becky Ben Jasper Lyla Sam
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- A cystic-fibrosis mutation in a certain pedigree is due toa single nucleotide-pair change. This change destroys anEcoRI restriction site normally found in this position.How would you use this information in counseling members of this family about their likelihood of being carriers? State the precise experiments needed. Assume thatyou find that a woman in this family is a carrier, and ittranspires that she is married to an unrelated man whoalso is a heterozygote for cystic fibrosis, but, in his case, itis a different mutation in the same gene. How would youcounsel this couple about the risks of a child’s having cystic fibrosis?When comparing evolutionary similarities between different genes within a gene family, it is usually more straightforward to compare genes by using the protein sequences of gene products rather than DNA sequences of the genes themselves. Explain why this is the case. (Cover 4 points)The line below depicts a DNA segment from a eukaryote. In this case, only the top, coding strand, is shown which is ok for our purposes (but remember that the other strand would also be present in DNAà but it is ok for us to ignore). This is very similar to a problem on the old tests. For each letter state the structure or the function of what occurs at that region. Letter A is Letter B is Letter C is Letter D is Letter E is Letter F is Letter G is To which region/letter would RNA Polymerase bind To which region/letter GTF bind Label the position of the start codon ATG and the stop codon TGA The picture below is for the question itself
- Please consider the figure below, parts A and B. A В Gene B Gene C Gene B Normal Gene A Chromosome 12 Normal Chromosome 1 Please consider figure A. a. Do these chromosomes come from a dividing or non-dividing cell? Give a reason for your answer. b. How many molecules of double stranded DNA will be present at anaphase I of a cell from this organism? Please consider figure B. A potentially carcinogenic mutation occurred on one of the chromosomes. The gene affected by the mutation codes for a protein involved in the repair of DNA damage. c. What is the correct term used to describe this chromosomal mutation? d. In terms of the development of cancer, is this a dominant or recessive mutation? e. What is the consequence of this mutation if it occurs during meiosis and is inherited by the offspring? Explain your answer in terms of the function of the protein.In Figure , what do the red and blue parts of the DNA labeled by balloon 6 represent?Familial retinoblastoma, a rare autosomal dominant defect, arose in a large family that had no prior history of the disease. Consider the following pedigree (the darkly colored symbols represent affected individuals): a. Circle the individual(s) in which the mutation most likely occurred. b. Is the person who is the source of the mutation affected by retinoblastoma? Justify your answer. c. Assuming that the mutant allele is fully penetrant, what is the chance that an affected individual will have an affected child?
- Which members of the pedigree could have been carriers, and which might have been the source of the mutation?The results of a paternity test are shown in the table below. Numbers indicate the number of short tandem repeats for loci tested. Whos the daddy? How sure are you?In Figure 14-10, expressed sequence tags (ESTs) arealigned with genomic sequence. How are ESTs helpful ingenome annotation?
- In the genotype presented (genomic plus plasmid genes), which of the following statements Is phenotype? genome: IS P O`z+Y° plasmid: I* Pt otzY+ no production of either B-gal and permease O B-gal and permease induced when lactose is present no B-gal is produced and expression of permease when lactose is present constitutive expression of B-gal and permease constitutive expression of B-gal and no expression of permeaseHere is a DNA agarose gel showing PCR products from a mouse genotyping experiment. Genotyping tells us whether each mouse is a wild type mouse (i.e. not genetically modified) or a mutant mouse. Interpret the results for each mouse 1-3.an orginal dna sequence retrieved from the gametes of a female golden retriever and a mutated dna sequence fro her puppy are found below. how will this mutation effect descentants of this puppy