Time point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain of Escherichia coli (E. coli). b. Estimate the doubling time for this strain of E. Coli. Clearly show how you estimated this value from the empirical data presented.
Time point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain of Escherichia coli (E. coli). b. Estimate the doubling time for this strain of E. Coli. Clearly show how you estimated this value from the empirical data presented.
Chapter14: Formula Method
Section: Chapter Questions
Problem 34SST
Related questions
Question
Time point (min) |
Absorbance of culture at 660nm |
Approximate cell concentration |
Approximate # cells in 1mL extract |
0 |
0.298 |
1.49 x 108 cells/mL |
1.49 x 108 cells |
10 |
0.316 |
1.58 x 108 cells/mL |
1.58 x 108 cells |
20 |
0.374 |
1.87 x 108 cells/mL |
1.87 x 108 cells |
30 |
0.429 |
2.145 x 108 cells/mL |
2.145 x 108 cells |
40 |
0.512 |
2.56 x 108 cells/mL |
2.56 x 108 cells |
50 |
0.544 |
2.72 x 108 cells/mL |
2.72 x 108 cells |
60 |
0.607 |
3.035 x 108 cells/mL |
3.035 x 108 cells |
a. Using these data, prepare a growth curve of this strain of
Escherichia coli (E. coli).
b. Estimate the doubling time for this strain of E. Coli. Clearly show
how you estimated this value from the empirical data presented.
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