VI. Four compounds were analyzed on tlc plate after spotting them and developing them with 75% Ethyl acetate in hexane: Distance travelled by the solvent is: 8.0 cm Distance travelled by Compound A is: 6.5 cm Distance travelled by compound B is: 4.85 cm Distance travelled by compound C is: 2.85 cm Distance travelled by Compound D is 1.022 cm Calculate the Rf values of A, B, C and D Rf value of A: Rf value of B: Rf value of C: Rf value of D:
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- Absorbance In an analysis of herbal medicinal compounds, Glycyrrhizin, cinnamic acid, and cinnamaldehyde are analyze in UV-vis absorption spectrometer indiviually (individual spectra shown in Fig 2), and a mixture of the three compounds are separated in HPLC (conditions given in Fig 3 caption), monitored with two UV detectors at 254 nm and 285 nm (chromatograms in Fig 3). Wavelength Glyamhien marker compounds. 2 ppm: cimuamaldehyde. 2 ppm. Cinnamic acid HO HOOG HO НО Cinnamaldehyde Figure 2 The photodiode array UV spectra of Chinese herbal medicine glycyrrhizin. 10 ppm: cinnamic acid. HOOC HO Q OH OH Absorbance MAU 10- 88 0 Figure 3. ثلث 10 B B 12 C Retention time (min) 254 nm 285 nm 18 Gradient separation of a mixed standard solution containing glycyrrhizin. cinnamic and cinnamaldehyde at concentration of 10, 2 and 2 ppm respectively. Conditions: Zorbax SB-C18. 4.6 x 12.5 mm guard column + 4.6 x 250 mm analytical column, 5-um dp: ACN: 25mM phosphate buffer pH 3.0. 1 mL/min: 20 °C: inj…Question 2 Which amomg the fragments below will be dectected by mass Spectrophotometer? i. [CH3CH31* ii. CH3CH3 iii. -CH2CH3 iv. [CH3CH4] only i ii and iv only i O i and iii only i5. Properties and IMF: Chlorophyll a and Chlorophyll b are almost identical in structure, but they separated during TLC. Report the RFs you recorded for both molecules. What functional group(s) are different between them? Then explain these RF values by comparing the IMF present in each molecule to those present in the mobile or stationary phase and how this shaped their affinities for each phase. Use the terms RF, IMF, polar, nonpolar, affinity, mobile phase and stationary phase.
- Calculate the Rf value from the information given for both the known and unknown solutions and identify each unknown solution based on the Rf values from the known solutions. distance the spottraveled Rf = %3D distance the solvent traveled Distance the solvent travels: 6.1cm Known Solutions Acetaminophen in Ethanol: traveled 3.4 cm 2% Ibuprofen in Ethanol: traveled 5.2 cm 1% Caffeine in Ethanol: traveled 1.1 cm 2% Aspirin in Ethanol: traveled 3.5 cm Unknown Solutions Unknown 1: traveled 3.2 cm Unknown 2: traveled 3.7 cm Unknown 3: traveled 1.0 cm Unknown 4: traveled 4.9 cm Questions: In Thin-Layer Chromatography, what is the stationary phase? What is the mobile phase? 2. This diagram from the chemguide page shows the results of a thin layer chromatography experiment. A. Describe briefly, but precisely what would have been done to get to this stage. You can assume that you have height reached by he solvent the "salvent ton been given a suitable thins layer chromatography plate. В. Why…1.0 g of a compound A is prepared in 100 mL of water, and then extracted with 50 mL of ether. After the extraction, 0.25 g of the compound was recovered from the ether layer. What is the partitioning coefficient([A]ether/[A]water) for this compound in the system used? 50 ml ether 0g 0.25 g 1g 100 ml water O 4.0 O 3.0 O 1.5 O 0.75 O 0.25 O 0.67CI NH 'N' chloroquine CI NH LHO N. N: hydroxychloroquine 5. You attempted to separate chloroquine and hydroxychloroquine using paper chromatography. You used a mixture of benzene and pyridine as your solvent. What results can you expect? O Two bands: hydroxychloroquine will have a higher Rf than chloroquine One band: Both will have equal Rf Four bands: The enantiomers of each analyte will appear as separate bands Two bands: chloroquine will have a higher Rf than hydroxychloroquine
- 2. A student spots a mixture of the following compounds on a TLC plate and uses a 50:50 mixture of hexanes: ethyl acetate as the development solvent. Which compound is expected to have a larger Rf value? но and A В Not enough information given to answer the question. Their structures are too similar to separate by TLC. O AQ1. Why is it important to acquire a reference spectrum and subtract it from the sample’s spectrum? Q2. Why are quartz cuvettes better for UV-visible absorption spectroscopy than polystyrene cuvettes, especially at lower wavelengths (UV)? Q3. What would happen if you put too much of a compound in the cuvette during the spectral measurement? Q4. Why is it important to avoid any scratch on the cuvette? Q5. Could you use a compact fluorescent light (CFL) bulb to perform UV-visible absorption spectroscopy? Why/why not? Q6. Predict the maximum absorption wavelength for 2,4-heptadien-6-one using Woodward-Fieser rules. Q7. Why are n-π* transitions generally of low intensities/low absorptions? Q8. What photon energy and wavelength would you need to send on a compound having an energy gap (Eg) of 2.7 eV? Detail your answer with appropriate information. Q9. Using molecular orbital theory/concept (draw the important molecular orbitals), explain the difference in absorption for the…1. Answer true or false for each statement: a) A TLC analysis is sufficient to characterize the purity of a compound. b) Carboxylic acids containing six or more carbon atoms per molecule are more soluble in2.5 M sodium hydroxide than in diethyl ether. c) Phenols containing six or more carbon atoms per molecule are more soluble in 2.5 M sodium hydroxidethan in diethyl ether. d) Benzoic acid and p-nitroaniline form water-soluble salts, whereas naphthalene does not. e) TLC analysis is the method of choice to determine the type of solid phase and eluent system that is required for a column chromatography separation. f) Mineral oil is an inappropriate heating fluid for determining the boiling points of samples that exceed 200°C (760 torr)? g) A heating bath containing mineral oil should not be used to determine the melting points of solids meltingabove 200 °C
- Compound X was developed on a TLC plate using a given eluent. The compound had a Rf value of 0.93 and the solvent front is 10.1 cm from the origin. Using this information, calculate the distance in cm that compound X traveled from the origin. Report your answer to one decimal place. cmHow would you discuss method of separation and identification of compounds in TLC?19. Explain the peaks in the following mass spectra: a. 2-pentanone: m/e 86, 71, 57, 43 (100 %) b. 1,2-dichloroethane: m/e 100, 98, 64, 62 (100 %), 51, 49, 27 c. Bromocyclohexane: m/e 163, 161 (both very small), 83 (100 %), 55, 41.