For an enzyme that follows Michaelis –Menten kinetics, k1=1X106 M-1 sec-1, k-1= 2x103 sec-1 and k2=2X 102 sec-1. a. What is the Km for the enzyme ? If the enzyme concentration is 10 nanomoles in 1 ml, what is Vmax for the enzyme ? What is the catalytic efficiency for this enzyme?

To calculate the Michaelis Menten constant Km. In enzymatic reaction the enzyme act as the…

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What is the Km for the enzyme

Chemistry for Today: General, Organic, and Biochemistry

9th Edition

ISBN:9781305960060

Author:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen

Publisher:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen

Chapter20: Enzymes

Section: Chapter Questions

Problem 20.2E: List two ways that enzyme catalysis of a reaction is superior to normal conditions.

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Question

For an enzyme that follows Michaelis –Menten kinetics, k1=1X10⁶ M^-1 sec^-1, k-1= 2x10³ sec^-1 and k2=2X 10² sec^-1. a. What is the Km for the enzyme ?

1. If the enzyme concentration is 10 nanomoles in 1 ml, what is Vmax for the enzyme ?

1. What is the catalytic efficiency for this enzyme?

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