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- 24. On the basis of your knowledge of osmosis, predict what will happen to the water in Elodea cells.3. Paramecia are unicellular organisms that have contractile vacuoles to remove (pump) excess intracellular water. In an experimental investigation, these paramecia were placed in salt solutions of increasing concentration. The rate at which the contractile vacuole contracted to pump out excess water was determined and plotted against osmolarity of the solutions (increased osmolarity = higher salt concentration), as shown in the graph. What is the best explanation for the data? Osmolarity of Solution (mM) Your answer Rate of Contraction (contractions per minute)10. What is osmosis? Is it correct to call it the diffusion of water across a membrane permeable only to the solvent, (water), and impermeable to the solutes? Define and understand the terms: isotonic, hypotonic, hypertonic. What are the these conditions on cells with or without cell walls? consequences of
- 8. Phosphate buffered saline (PBS) is often used as an isotonic solution to keep cells happy and alive. Though recipes vary, PBS typically contains 157 mM Na* and 4.5 mM K+, yet inside of the cell that ratio is switched (there is approximately 150 mM K* and only about 10 mM Nat). Why is PBS still considered isotonic? That is, why does putting cells into PBS not create a lot of osmotic pressure?3. Draw and label a diagram that demonstrates the difference between tonicity and osmolarity. Be sure to label the new solutions in each condition, draw the changes (if predicted) in cell shape/volume, and clearly use arrows to indicate the movement of water relative to the cell. Please start with a red blood cell in an isotonic solution (Figure D) and show what happens to cell shape and water movement when NaCl (remember that the membrane is permeable to neither of these ions) (Figure E) or urea (a permeable ion) (Figure F).1. in what direction water flows by osmosis through a semipermeable membrane when placed in a hypotonic solution, isotonic, or hypertonic environment? 2. Animal Cells burst or lyse in an environment?
- 28. What could be inferred about the level of concentration of the solution inside and outside of the cell in Solution C before any movement of water had taken place? A. The percentage of solution concentration inside and outside the cell is equal B. The percentage of solution concentration inside and outside the cell is constant. C. The percentage of solution concentration inside the cell is greater than outside the cell D. The percentage of solution concentration inside the cell is lesser than outside the cell1.How will a plant cell that is subjected to a solute concentration that is less than that of inside the cell will react 2.What is the importance of the concentration gradient in passive transport?18.. Use the following diagram to answer the questions. Assume the membrane is semipermeable to glucose, water, and iodine (IKI). A. Which solutes will diffuse into the cell? B. Which solute will diffuse out of the cell? 10% glucose 40% glucose C. Which solute will not diffuse at all? 20% starch 0% starch D. What type of solution (the outside of the cell - hypertonic, isotonic or hypotonic) does the diagram represent? 70% water 50% water 10% IKI E. What color will the cell be at the conclusion of this experiment? What color will the solution in the beaker be? Justify your answer,
- 4. A researcher is interested in the mode of transport of three different substances (A, B, & C) across the cell membrane. The researcher's data is presented in the graphs below. A) A Initial rate of transport into the Bor C cell Concentration The graph above shows the initial rate of transport for each substance when cells that do not contain A, B, or C are placed in solutions of differing concentrations of A, B, and C, respectively. Based on this data, which of the following is/are compatible modes of transport for substance A? (active transport, facilitated diffusion, simple diffusion) For substance B? For substance C?Thank you4. The normal osmolarity and glucose level of plasma are 300mmol/L and 1g/L, respectively. Estimate the difference between the osmotic pressure of the plasma of a diabetic patient, who has a glucose level of 8g/L, and a patient that has a normal glucose level. Provide the result in atmospheres and meters of water. Ignoring possible physiological compensation mechanisms, what would you expect to happen to the body cells? How would the cells compensate if they could?