You are planning to run a gel using a Tris-HCl buffer. You have 0.75 M Tris-HCl, pH 7.30 buffer, but you need your final buffer to be at a concentration of 100 mM and you need a total volume of 1.5 liter. What do you do? (Remember C1 V1 = C2 V2). How much water needs to be added, too?
You are planning to run a gel using a Tris-HCl buffer. You have 0.75 M Tris-HCl, pH 7.30 buffer, but you need your final buffer to be at a concentration of 100 mM and you need a total volume of 1.5 liter. What do you do? (Remember C1 V1 = C2 V2). How much water needs to be added, too?
Principles of Modern Chemistry
8th Edition
ISBN:9781305079113
Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Chapter15: Acid–base Equilibria
Section: Chapter Questions
Problem 95AP
Related questions
Question
You are planning to run a gel using a Tris-HCl buffer. You have 0.75 M Tris-HCl, pH
7.30 buffer, but you need your final buffer to be at a concentration of 100 mM and you need
a total volume of 1.5 liter. What do you do? (Remember C1 V1 = C2 V2). How much water
needs to be added, too?
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 2 steps with 2 images
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.Recommended textbooks for you
Principles of Modern Chemistry
Chemistry
ISBN:
9781305079113
Author:
David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:
Cengage Learning
Principles of Modern Chemistry
Chemistry
ISBN:
9781305079113
Author:
David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:
Cengage Learning