You have just transformed 1 ul (100 pg/ul) of control pWasabi (plasmid) DNA into 50 ul of E. coli DH50a by the heat-shock method. You then add 950 µl of SOC medium to outgrow the bacteria. Of this, you plated 50 µl onto a LB plate containing ampicillin. After overnight incubation, you observed that there are 150 colony forming units (cfu) on the plate. Calculate the transformation efficiency (show your step-wise calculations below).
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Step by step
Solved in 3 steps with 4 images