You utilised two plasmids in this practical, pOTC and pOTC-Δ. Plasmids are often represented using plasmid maps like the one below. This map shows the positions of recognition sites for a number of restriction enzymes Using the plasmid map of pBCH2.0 provided above, predict how many DNA fragments would be formed if this plasmid was digested with restriction enzymes EcoRI and PvuII.

Q: QUESTIONS: 1. Explain why a vegetable and fruit diet alkalinize blood pH. 2. What are the four major…

A: Diet is a combination of substances that we take throughout a day. These can be a combination of…

Q: Comparing the active site geometries of chymotrypsin and subtilisin enzymes under the hypothesis…

A: Chymotrypsin : it is a very powerful proteolytic(protein breakdown enzyme) which is being secreted…

Q: Which of the following is/are (a) method(s) for identifying proteins?   A  ELISA B  Eastern blot…

A: Proteins are unbranched polymers constructed from 20 standard α-amino acids. They have four levels…

Q: Select the answer with an INCORRECT statement about the hydrophobic effect It is important for…

A: Hydrophobic effect is the tendency of non polar molecules to interact with each other in polar…

Q: Write TRUE or FALSE. If false, write the word/s that make(s) the statement incorrect. The 3D…

A: The 3D structure of ribosomal RNA / rRNA consists of a very complex pattern of stems and loops. The…

Q: Which of the following 1-C unit:carrier pairing is/are correct? a.biotin:C02 b.THF:-CH2-…

A: Biotin is a co-factor that carries CO2 and regulates the activities of enzymes like acetyl-CoA…

Q: Fatty acids Yield about: A. 7 ATP per C at B. 120 ATP per C atom C. 32 AIP C atom D. 14 ATP per C…

A: To obtain ATP from fatty acids, they are broken down to acetyl-CoA. This process is known as…

Q: An allosterically regulated enzyme in the R state is unable to bind substrate at the active site.…

A: Allosteric site : It is a place or site on an enzyme where the molecule which is not a substrate can…

Q: Gases such as N2 and O2 can diffuse simply across membranes but not small organic compounds as…

A: Diffusion: It is the process of movement of molecules from high concentration to low concentrations…

Q: cell division, what do you think is this other explanation for the loss of body fat in cancer…

A: Cancer patient when undergo chemotherapy for treatment have a feeling of nausea and headache along…

Q: Detail the function of amino group, carboxyl group and R group found in Tyrosine

A: Tyrosine is a non-essential amino acid that is coded by UAC and UAU. It is a protein target for…

Q: In this FRET data table, an antibiotic was added, but the type was not recorded. According to the…

A: FRET (Förster resonance energy transfer) is a technique in which energy transfer between two…

Q: (ER) fluids seen by naked eyes Material class: Material type [ non-smart/ smart / nano ] : Material…

A: A material is a substance that assumes a certain form under specified conditions. A material can be…

Q: Define Parfocal.

A: A microscope is an instrument used to make very small objects or things visible to the naked eye. It…

Q: CAN YOU PLEASE ANSWER 1b 1a) The phosphate groups in the sugar-phosphate backbone of each strand of…

A: DNA (deoxyribonucleic acid) is the nucleic acid that acts as the genetic material. Each strand of a…

Q: The retention factor, Rf is measured: Any place in a spot - some of the molecules migrated there. At…

A: Rf means retention factor in a thin layer of paper chromatography. A solute or mixed sample is…

Q: Primer Designing:

A: Primer desingning: Primers are short DNA sequences used to amplify the larger fragment of DNA in a…

Q: Galactosemia is due to?    A. lactase deficiency  B. accumulation of lactic acid C. accumulation…

A: Galactosemia is a metabolic disorder, due to the deficiency of the enzyme galactose-1-phosphate…

Q: The total concentration of enzyme in a reaction, [E], is made up of the concentration of enzyme…

A: Enzymes are the protein molecules that consist of active sites at which substrate molecules bind to…

Q: Calculate the amount of ATP that can be produced from one molecule of 8:0 fatty acid metabolized…

A: 8:0 fatty acid means its a 8 carbon saturated fatty acid with no carbon-carbon double bond. A 8:0…

Q: Which of the following is an example of a secondary active transport? a. CPT-I b. O2 pump c.…

A: Transport across the plasma membrane is classified as passive and active. Passive transport is the…

Q: Why does the velocity reach a plateau (asymptote) at high substrate concentrations?       a. At high…

A: Introduction Enzymes are known as biocatalyst. They increase rate of a chemical reaction by reducing…

Q: Assuming the oligopeptide ALPHAHELICKS forms one continuous α helix, the carbonyl oxygen of the…

A: Alpha-Helix is a type of secondary structure commonly found in proteins. Proteins are composed of…

Q: Give the structure and the corresponding function of Inulin.

A:

Q: A) Write out both strands of the recognition sequences for BamHI and Sau3A, show what they would…

A:

Q: n why a sugar can form at least two different glycosides.

A: Sugars an form two different  glycosides.

Q: Estimate the flux (mg/cm2/s) by diffusion of estrogen (a steroid) through a lipid bilayer cell…

A: Flux of diffusion represents the amount of substance that can moved in or out of the cell through a…

Q: list the amino acids that will carry a net charge at pH 7 within a protein. What is the charge at pH…

A: Note : Hi ! Thank you for the question. We are authorized to answer one question at a time. Since…

Q: A fully folded, functional single polypeptide is in ____________________ structure.

A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…

Q: What interactions stabilize the formation of a ß-sheet from ß-strands? a) Salt bridges from charged…

A: A protein's function depends on its structure. There are four levels of protein structure: primary,…

Q: Which of the following biomolecules is/are NOT amphipathic?

A: Amphipathic molecules are those molecules which has both polar and non-polar parts. 1) The polar…

Q: 8. Reversible precipitation of protei ns from aqueous solutions can be made under action of: A.…

A: Proteins are composed of amino acids. They are linked together by peptide linkages. Proteins have…

Q: Which of the following statements is/are TRUE for globular proteins? A. sensitive to changes in pH…

A: Globular proteins are a group of proteins that are spherical in shape and they are known to be water…

Q: 35. What is the approximate pl for the peptide MEKVDRP. Because this is a peptide, assume the pka…

A: Peptide : Peptides are short or small chains of amino acids having about 2-50 amoino acids. As amino…

Q: The sources of the three (3) carbons in malonyl-Coa is/are: a. 1 C from C02 and 2 C from acetyl-CoA…

A: Malonyl-CoA is one of the major molecules and are involved in fatty acid biosynthesis .

Q: give a clear handwritten answer only with explanation..Draw 5’-A-A-3’ ribonucleotide with 5’…

A: Ribonucleotides are made up of ribose sugar, a nitrogenous base and a phosphate. These are present…

Q: Functions of proteins in cells; How enzymes function as catalysts?

A: Chemical catalysts are substances that when added to a reaction increase the rate of reaction and do…

Q: The following are true statements regarding passive facilitated transport EXCEPT     The process…

A: Diffusion is a defined biological process by which molecules transport through organs. Diffusion is…

Q: Which of the following correctly describes the reactions in the β-oxidation cycle?   oxidation →…

A: Beta oxidation is a process in which fatty acids are broken down to produce energy from them .

Q: What are the relative percentage (%) of species (B) and (C) at pH 7.2? (Note: treat pka's as…

A: Amino acids contain an alpha-amino group, an alpha-carboxylic group, and a side chain. The side…

Q: 2.) An unknown amino acid was subjected to paper chromatography with five amino acid standards in…

A: Paper chromatography is a biochemical technique that is used to separate a mixture of substances…

Q: Electrically neutral molecules can pass through cell membranes more easily than charged molecules…

A: Cell membrane are composed of phospholipids, hence only electrically neutral molecules, smaller in…

Q: 14) Which of the following statements is true under the conditions provided: the enzyme…

A: For a one-substrate enzyme-catalyzed reaction, the Michaelis-Menton equation shows the quantitative…

Q: Which of the following are elongation factors involved in the release of free tRNAs? a.EF-G…

A: The above mentioned elongation factors are involved in the process of translation in prokaryotes.…

Q: Lysine is degraded to acetoacetyl-CoA then to acetyl-CoA, and is described as a ­­­­_______ amino…

A: Lysine and arginine are broken down into acetoacetyl CoA and Acetyl CoA respectively. The process…

Q: Determine the Primary amino acid sequence of an Octapeptide Composition · Tyr , Arg，Pro, Lys ,Lys…

A: Introduction Amino acids are building block of proteins. Amino acids contain both amino and…

Q: Which of the following is/are TRUE about the ETC? A  Complex IV: last e- acceptor is Cu2+   B…

A: The electron transport chain ( ETC) is a process in which the NADH and FADH2 produced during…

Q: Metabolite common between Krebs’ cycle and β-oxidation cycle? A. pyruvate B. acetyl-CoA C.…

A: Krebs cycle is the second stage of cellular respiration in which Acetyl CoA is harnessed for its…

Q: a. Synthesizing glutamine is endergonic and so not spontaneous. How would having hese two reactions…

A: Coupled bioenergetics reactions are set of chemical reaction in which energy is moved from one side…

Q: What ionization state of Glu35 and Asp52 side chain predominates at pH 1, 4, and 7?

A: Glutamate and Aspartate are negatively charged amino acid. At pH 7 Glu35 and Asp52 side chains have…