A gene knock-out can be described as: Question 2 options: Introduction of a cloned gene into a living cell in an attempt to cure a disease A genetic modification that allows control over when and where the gene is deleted Insertion of a gene into the genome Transgenic mice Complete removal of a gene in all cells within an organism

A gene knock-out can be described as: Question 2 options: Introduction of a cloned gene into a living cell in an attempt to cure a disease A genetic modification that allows control over when and where the gene is deleted Insertion of a gene into the genome Transgenic mice Complete removal of a gene in all cells within an organism

BIOLOGY:CONCEPTS+APPL.(LOOSELEAF)

10th Edition

ISBN:9781305967359

Author:STARR

Publisher:STARR

Chapter15: Biotechnology

Section: Chapter Questions

Problem 5SA: A set of cells that host various DNA fragments collectively representing an organisms entire set of...

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Being able to understand the relationship between DNA, mRNA, tRNA, ribosomes and proteins, is necessary for any researcher interested in gene therapy. Gene therapy is dedicated to correcting genetic defects by replacing mutated genes with wildtype versions. This can include complete removal and introduction of a working copy of the entire gene (using knock-outs and knock-ins) or removal of only the actual mutated nucleotide. Sickle Cell Disease is an example of a recessive disorder based on a single-point mutation: GAG becomes GTG, which encodes a new amino acid. One would only need to replace a single base to correct the disorder making it very attractive to gene therapy researchers. The mutant sickle cell allele is called HbS (for sickled hemoglobin) while the wt allele is HbA. Pretend that Arcel Kasongo, a masters student from Cambridge, is studying for an oral exam on gene therapy, and has an especial interest in the disorder since he comes from the Democratic Republic of Congo…
Fill in the following with the correct words about cloning (the first blank will be the first word and the second blank will be the second word. There are two types of cloning, the first is when scientists change the cellular function this is called cloning and can help with creating insulin and vaccines to name a few. The other type is when DNA is extracted from a somatic cell and is used to replace DNA in an egg, this is called cloning. O gene, organism O organism, gene O specific, general O micro, macro
Genetic Engineering Terms Name: Draw a line to connect each pair of boxes DNA sequencing The use of an organism, or a component of an organism or other biological system, to make a product or process. DNA cloning The sequencing, analysis, and cutting-and-pasting of DNA A technique to make many copies of a specific DNA region in vitro (in a test tube rather than anorganism) Recombinant DNA Polymerase chain reaction A technique used.to separatę DNA fragments according to their size Gel electrophoresis DNA that is assembled out of fragments from multiple sources Biotechnology A molecular biology technique that makes many identical copies of a piece of DNA, such as a gene DNA technology The process of determining the sequence of nucleotides (As, Ts, Cs, and Gs) in a piece of DNA. ww tİ6111749/enetic-engineering-tems
Fill the Table with mutagenic agents and provide their type (physical, chemical, biological) and their classification based on their effect (teratogenic, carcinogenic, clastogenic, or non-specific), together with their modes of action. Mutagen Type of Mutagen Classification based on effect Action UV – radiation X-Ray radiation Virus Heat 5-bromouracil Ethidium bromide 2-aminopurine Acridine orange Proflavine Cobalt Nickel Methylhydrazine Temozolomide Ethyl ethane sulfate
The enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy. Explain what they are used for. Also mention the actual biological function of the respective enzymes. T7 RNA polymerase reverse transcriptase RNaseH
Below are some of the arguments about the use of transgenic organisms. From your own perspective, explain your answer in the questions in not more than 5 sentences. 1. If you are a farmer would you take the chance of growing crops that are pest resistant? Why or why not? 2. Considering the knowledge gained in genetic engineering, would you try to patronize GMO fruits and vegetables? Why or why not? 3. Is creating or altering genes of an organism a form of Blasphemy to the creator (God)? Why? 4. Is genetic engineering morally permissible or not?
Make a list of ethical considerations for using gene therapy.
Gene therapy involves: a. the introduction of recombinant proteins into individuals b. cloning human genes into plants c. the introduction of a normal gene into an individual carrying a mutant copy d. DNA fingerprinting e. none of these
You are a genetic counselor, and your patient has asked to be tested to determine if she carries a gene that predisposes her to early-onset cancer. If your patient has this gene, there is a 50/50 chance that all of her siblings inherited the gene as well; there is also a 50/50 chance that it will be passed on to their offspring. Your patient is concerned about confidentiality and does not want anyone in her family to know she is being tested, including her identical twin sister. Your patient is tested and found to carry a mutant allele that gives her an 85% lifetime risk of developing breast cancer and a 60% lifetime risk of developing ovarian cancer. At the result-disclosure session, she once again reiterates that she does not want anyone in her family to know her test results. a. Knowing that a familial mutation is occurring in this family, what would be your next course of action in this case? b. Is it your duty to contact members of this family despite the request of your patient? Where do your obligations lie: with your patient or with the patients family? Would it be inappropriate to try to persuade the patient to share her results with her family members?
A set of cells that host various DNA fragments collectively representing an organisms entire set of genetic information is called a _______ . a. genome c. genomic library b. clone d. GMO
The Human Genome Project attempts to modify organism’s DNA inserting desired genes into bacteria. true or false
Fill the Table with mutagenic agents and provide their type (physical, chemical, biological) and their classification based on their effect (teratogenic, carcinogenic, clastogenic, or non-specific), together with their modes of action. Mutagen Type of Mutagen Classification based on effect Action Heat 5-bromouracil Ethidium bromide

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	Introduction of a cloned gene into a living cell in an attempt to cure a disease
	A genetic modification that allows control over when and where the gene is deleted
	Insertion of a gene into the genome
	Transgenic mice
	Complete removal of a gene in all cells within an organism