Select any and all statements about polymerase chain reaction (PCR) that are true. There may be more than one answer.PCR allowed for the quick production of millions of copies of DNA sequences in a short timeOPCR uses an enzyme called taq polymerasePCR allows us to edit genes through single nucleotide exchangeOPCR was used to make one complete copy of the entire human genomeOPCR relies on reverse RNA transcription

Polymerase Chain Reaction (PCR) is an enzymatic process in which a specific region of DNA is…

Answered: Select any and all statements about…

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 15QP

See similar textbooks

Similar questions

Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymerase
Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.
Cloning Genes Is a Multistep Process The following DNA sequence contains a six-base sequence that is a recognition and cutting site for a restriction enzyme. What is this sequence? Which enzyme will cut this sequence? (See Figure 13.5 for help.) 5 CCGAGGAAGCTTAC 3 3 GGCTCCTTCGAATG 5
Which ingredient will allow the DNA to form cloudy clumps which can be collected with tweezers salt rubbing alcohols detergent solution water What is the purpose of PCR? To copy and then make many copies of a specific region of DNA To copy the entire human genome To make just a few copies of DNA To reveal the sequence of a piece of DNA If enzyme catalase has an optimum pH of 7.0, What do you think will happen en the pH is lowered to 3.0? The rate of its enzyme action is increased. Its enzyme action is not affected at all. The rate of its enzyme action is decreased. Its enzyme action ceases or stops.
What purpose do short DNA primers serve in PCR? O They determine which sequence of DNA is copied O They synthesize new DNA O They synthesize proteins O They separate RNA from DNA MacBook Air 80 888 F7 F6
You want to amplify a specific region of DNA during PCR. Your primers should a Both should be an exact replica of the beginning of the specific gene you want to amplify b Both should be complementary to the beginning of the specific gene you want to amplify c One primer should be identical to the beginning of the gene sequence while the other is identical to the end of the gene sequence d One primer should be complementary to the beginning of the gene sequence while the other is complementary to the end of the gene sequence e One primer should be identical to the beginning of the gene sequence while the other is complementary to the end of the gene sequence
What type of enzyme would you need to insert a functional gene in a particular part of the genome? A nuclease that makes staggered cuts A polymerase that recognizes particular DNA sequences A nuclease that makes blunt cuts A polymerase that can copy the required sequence
Suppose that you want to clone a gene that has never been analyzed before by DNA sequencing. Which of the following is the most accurate? O Perform a PCR to clone the gene because it is much faster. Perform a PCR to clone the gene because it is very specific and gives a high yield. O PCR cannot be performed because the DNA sequence is not known yet so the forward and reverse primers cannot be designed. O PCR can be performed because although the DNA sequence of the sample is not known yet, an available primer can still be used.
Explain the role of each of the two enzymes used in the room temperature -PCR process. Reverse transcriptas Taq DNA polymerase
Why is DNA polymerase a required part of PCR? O It synthesizes new DNA O It synthesizes proteins O It degrades the template DNA O It separates RNA from DNA MacBook Air
Briefly describe in your own words what DNA barcoding is. Explain how PCR is used in this process.
Biotechnology There are several different tools used to study or manipulate DNA. Match the description to the technology. NOTE: If you want to change your selection, you'll need to delete the one you already chose. After you delete it, the list of choices will pop back up and you can make a different choice. Polymerase Chain Reaction (PCR) Replacing defective genes that caused a disease Gel Electrophoresis Circular DNA used to insert genes Molecular cloning Cuts DNA into smaller pieces Tool for gene editing Restriction enzyme Creates larger quantities of DNA Plasmids Produces genetically identical DNA fragments Separates fragments of DNA by size CRISPR Gene therapy

SEE MORE QUESTIONS

Recommended textbooks for you

Human Heredity: Principles and Issues (MindTap Co…

Biology

ISBN:

9781305251052

Author:

Michael Cummings

Publisher:

Cengage Learning

Biology Today and Tomorrow without Physiology (Mi…

Biology

ISBN:

9781305117396

Author:

Cecie Starr, Christine Evers, Lisa Starr

Publisher:

Cengage Learning

Human Heredity: Principles and Issues (MindTap Co…

Biology

ISBN:

9781305251052

Author:

Michael Cummings

Publisher:

Cengage Learning

Biology Today and Tomorrow without Physiology (Mi…

Biology

ISBN:

9781305117396

Author:

Cecie Starr, Christine Evers, Lisa Starr

Publisher:

Cengage Learning

SEE MORE TEXTBOOKS