1- To generate a sequencing library of transcriptome, the cellular    is converted to a                    library followed by                                and ligation of                          . A defined                    range of the fragments are further purified for the following high throughput

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1- To generate a sequencing library of transcriptome, the cellular    is converted to a                    library followed by                                and ligation of                          . A defined                    range of the fragments are further purified for the following high throughput                               .

2- To identify genes from sequencing data, read sequences are either ___________  to a reference genome or by de novo _____________.

3-  is a normalization procedure of read counts data from sequencing, which the                     of read counts of a transcript is adjusted for the                         of the transcript and by                                               in a sequence run.

4- To identify differentially expressed genes using whole transcriptome analysis, it is essential to test for ____________ significance with ____________ corrections of genomic data.

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