design a method to treat the most common mutation that causes sickle cell anemia using CRISPR/Cas. This mutation is a loss of function missense mutation that changes a glutamic acid to a valine in the beta-globin coding sequence. You remove from a patient and use a virus to insert which of the following sequences into the stem cells? utated gene at matches the mutated gene th wild type DNA sequence th mutant DNA

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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You would like to design a method to treat the most common mutation that causes sickle cell anemia using CRISPR/Cas. This mutation is a loss of function missense mutation that changes a glutamic acid to a valine in the beta-globin coding sequence. You remove
blood stem cells from a patient and use a virus to insert which of the following sequences into the stem cells?
i) Cas9 gene
ii) RNAI for the mutated gene
iii) Guide RNA that matches the mutated gene
iv) Donor DNA with wild type DNA sequence
v) Donor DNA with mutant DNA
o i, ii & iv
i, ii & iii
o i, iii & v
o i, ii, & iv
o i, ii, iii, iv & v
Transcribed Image Text:You would like to design a method to treat the most common mutation that causes sickle cell anemia using CRISPR/Cas. This mutation is a loss of function missense mutation that changes a glutamic acid to a valine in the beta-globin coding sequence. You remove blood stem cells from a patient and use a virus to insert which of the following sequences into the stem cells? i) Cas9 gene ii) RNAI for the mutated gene iii) Guide RNA that matches the mutated gene iv) Donor DNA with wild type DNA sequence v) Donor DNA with mutant DNA o i, ii & iv i, ii & iii o i, iii & v o i, ii, & iv o i, ii, iii, iv & v
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