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- Define disinfection.
- Define sterility.
- Compare and contrast disinfection with sterility.
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- In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperOther than the application of heat, name other methods of sterilization. Define the methods and give examples of materials sterilized in each method.Define sterilization.
- Define disinfection. Compare/contrast it with sterilization, antisepsis and bacteriostasis. Give the modes of action of the different antiseptics/disinfectants used in the activity (5% sodium hypochlorite (bleach), 0.05% sodium hypochlorite (bleach), 70% alcohol, 40% alcohol, Iodophor (Betadine), and Mouthwash)Disinfectants and Sterilization Procedures Complete the table by putting (+) for growth of microorganisms or (–) absence of growth of microorganisms. Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes 15 minutes Sodium hypochlorite 5% Sodium hypochlorite 0.05% Alcohol 70% Alcohol 40% Iodophor (Betadine) 10% solution Mouthwash Cetylpyridinium Chloride - 0.075% Sodium Fluoride - 0.05%Describe situations that require high-level disinfection and thosefor which medium- or low-level disinfection is adequate.
- Distinguish between the term’s sterilization, disinfection, antiseptics, and decontamination. Describe the circumstances in which these terms may be used.What are the BMET's role, protocol, and procedure for proper cleaning and or disinfecting equipment? Explain the different categories that determine when equipment must be disinfected or sterilized. Identify a common cleaning and disinfectant solution and the specific purpose of each.What is the difference between a disinfectant and an antiseptic? Which is most effective at removing microbes from a product: sanitization, degerming, or sterilization? Explain